A PCR Study using Tissue Microdissection Technique in Formalin-fixed, Paraffin-embedded Specimens
نویسندگان
چکیده
منابع مشابه
PCR assays detect B-lymphocyte clonality in formalin-fixed, paraffin-embedded specimens of classical hodgkin lymphoma without microdissection.
Hodgkin lymphoma (HL) was shown to be a B-cell malignancy using polymerase chain reaction (PCR) clonality studies of microdissected Reed-Sternberg cells. While methods for the detection of B-cell clonality could aid in the diagnosis of HL, microdissection is not practical in most clinical settings. We assessed the standardized BIOMED-2 IGH and IGK PCR primers for the detection of clonality usin...
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Archival tissue specimens are valuable resources of materials for molecular biological analyses in retrospective studies, especially for rare diseases or those associated with exposure to uncommon environmental events. Although successful amplification with PCR is essential for analysis of DNA extracted from archival formalin-fixed, paraffin-embedded (FFPE) tissue specimens, we have often encou...
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ژورنال
عنوان ژورنال: Journal of Clinical Otolaryngology Head and Neck Surgery
سال: 1997
ISSN: 1225-0244,2713-833X
DOI: 10.35420/jcohns.1997.8.1.78